Open AccessTruncated Newton's optimization scheme for absorption and fluorescence optical tomography: Part II Reconstruction from synthetic measurements
Author(s) -
Ranadhir Roy,
Eva M. SevickMuraca
Publication year - 1999
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.4.000372
Subject(s) - fluorescence , fluorophore , absorption (acoustics) , optics , materials science , contrast (vision) , inverse problem , excitation , diffuse optical imaging , tomography , quenching (fluorescence) , fluorescence lifetime imaging microscopy , physics , mathematics , mathematical analysis , quantum mechanics
Using two dimensional synthetic frequency-domain measurements, the inverse imaging problem is solved for absorption and fluorescence lifetime mapping with the truncated Newtons optimization scheme developed in Part I of this contribution. Herein, we present reconstructed maps of absorption owing to a fluorophore from excitation and emission measurements which detail the presence of tissue heterogeneities characterized by tenfold increase in fluorescent contrast agent. Our results confirm that fluorescence provides superior mapping of heterogeneities over excitation measurements. Using emission measurements we then map fluorescent lifetime under conditions of tenfold uptake of contrast agent in tissue heterogeneities. The ability to map fluorescent quenching and lengthening of contrast agents facilitates the solution of the inverse problem and further improves the ability to reconstruct tissue heterogeneities.