Open AccessHigh-speed chemical imaging of dynamic and histological samples with stimulated Raman micro-spectroscopy
Author(s) -
Xavier Audier,
Nicolas Forget,
Hervé Rigneault
Publication year - 2020
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.390850
Subject(s) - raman spectroscopy , optics , chemical imaging , materials science , spectral imaging , microscopy , imaging spectroscopy , spectroscopy , signal to noise ratio (imaging) , signal (programming language) , nuclear magnetic resonance , hyperspectral imaging , physics , computer science , quantum mechanics , artificial intelligence , programming language
We report a shot noise limited high-speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over 200 cm -1 in 12 µs at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters, the designed low noise imaging platform performs chemical imaging of dynamical processes such as Mannitol crystal hydration and reaches a signal to noise ratio sufficient to perform label free histological imaging on frozen human colon tissue slides.