Open AccessTwo-dimensional random access multiphoton spatial frequency modulated imaging
Author(s) -
Alyssa M. Allende Motz,
John Czerski,
Daniel E. Adams,
Charles G. Durfee,
Randy A. Bartels,
Jeffrey J. Field,
Christopher L. Hoy,
Jeff Squier
Publication year - 2020
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.378460
Subject(s) - optics , spatial light modulator , spatial frequency , phase modulation , excitation , random access , spectral imaging , modulation (music) , compensation (psychology) , amplitude , physics , computer science , acoustics , phase noise , psychology , quantum mechanics , psychoanalysis , operating system
Spatial frequency modulated imaging (SPIFI) enables the use of an extended excitation source for linear and nonlinear imaging with single element detection. To date, SPIFI has only been used with fixed excitation source geometries. Here, we explore the potential for the SPIFI method when a spatial light modulator (SLM) is used to program the excitation source, opening the door to a more versatile, random access imaging environment. In addition, an in-line, quantitative pulse compensation and measurement scheme is demonstrated using a new technique, spectral phase and amplitude retrieval and compensation (SPARC). This enables full characterization of the light exposure conditions at the focal plane of the random access imaging system, an important metric for optimizing, and reporting imaging conditions within specimens.