SIMPLE: Structured illumination based point localization estimator with enhanced precision | Zendy

Loïc Reymond | Zendy; J. F. Ziegler | Zendy; Christian Knapp | Zendy; Fung-Chen Wang | Zendy; Thomas Huser | Zendy; Verena Ruprecht | Zendy; Stefan Wieser | Zendy

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SIMPLE: Structured illumination based point localization estimator with enhanced precision

Author(s) -

Loïc Reymond,

J. F. Ziegler,

Christian Knapp,

Fung-Chen Wang,

Thomas Huser,

Verena Ruprecht,

Stefan Wieser

Publication year - 2019

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.27.024578

Subject(s) - optics , estimator , centroid , simple (philosophy) , physics , spatial light modulator , point spread function , microscopy , computer science , algorithm , artificial intelligence , mathematics , statistics , philosophy , epistemology

We present a structured illumination microscopy based point localization estimator (SIMPLE) that achieves a 2-fold increase in single molecule localization precision compared to conventional centroid estimation methods. SIMPLE advances the recently introduced MINFLUX concept by using precisely phase-shifted sinusoidal wave patterns as nanometric rulers for simultaneous particle localization based on photon count variation over a 20 μm field of view. We validate SIMPLE in silico and experimentally on a TIRF-SIM setup using a digital micro-mirror device (DMD) as a spatial light modulator.

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