Open AccessSIMPLE: Structured illumination based point localization estimator with enhanced precision
Author(s) -
Loïc Reymond,
Johannes Ziegler,
Christian Knapp,
Fung Chen Wang,
Thomas Huser,
Verena Ruprecht,
Stefan Wieser
Publication year - 2019
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.27.024578
Subject(s) - optics , estimator , centroid , simple (philosophy) , point spread function , physics , spatial light modulator , computer science , microscopy , algorithm , artificial intelligence , mathematics , philosophy , statistics , epistemology
We present a structured illumination microscopy based point localization estimator (SIMPLE) that achieves a 2-fold increase in single molecule localization precision compared to conventional centroid estimation methods. SIMPLE advances the recently introduced MINFLUX concept by using precisely phase-shifted sinusoidal wave patterns as nanometric rulers for simultaneous particle localization based on photon count variation over a 20 μm field of view. We validate SIMPLE in silico and experimentally on a TIRF-SIM setup using a digital micro-mirror device (DMD) as a spatial light modulator.