Open AccessRegulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy
Author(s) -
Chao-Mao Hsieh,
Patricia Yang Liu,
Lip Ket Chin,
Jing Bo Zhang,
Kuan Wang,
Kung-Bin Sung,
Wee Ser,
Tarik Bourouina,
Yamin LeprinceWang,
Ai Qun Liu
Publication year - 2019
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.27.022994
Subject(s) - lipid droplet , lipid metabolism , degree of unsaturation , raman spectroscopy , biophysics , lipidomics , microscopy , fatty acid , chemistry , materials science , optics , biochemistry , biology , chromatography , physics
Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes.