Open AccessFull fiber-optic fluorescence correlation spectroscopy
Author(s) -
Johtaro Yamamoto,
Masataka Kinjo
Publication year - 2019
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.27.014835
Subject(s) - optics , fluorescence correlation spectroscopy , materials science , optical fiber , dichroic glass , lens (geology) , fiber optic sensor , fluorescence , fluorescence spectroscopy , dichroic filter , fiber , spectroscopy , laser , optoelectronics , physics , quantum mechanics , composite material
A full fiber-optic fluorescence correlation spectroscopy (FF-FCS) technique has been developed without the use of objectives and dichroic mirrors. To achieve this, an excitation laser has been focused onto a sample by a lensed optical fiber or a gradient index lens attached on the terminal surface of the optical fiber. The FF-FCS system does not exhibit a higher sensitivity than the conventional FCS system; however, it is much simpler and smaller. This work demonstrates the feasibility of FF-FCS by measuring fluorescent beads. In the future, we expect FF-FCS to be widely used as a laboratory tool and an embedded tool for quality-control systems, such as cytometers.