Open AccessComputational correction of spatially variant optical aberrations in 3D single-molecule localization microscopy
Author(s) -
Ting Yan,
Charles J. Richardson,
M. Zhang,
Andreas Gahlmann
Publication year - 2019
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.27.012582
Subject(s) - optics , microscopy , point spread function , super resolution microscopy , microscope , optical microscope , physics , detector , photoactivated localization microscopy , common emitter , optoelectronics , scanning confocal electron microscopy , scanning electron microscope
3D single-molecule localization microscopy relies on fitting the shape of point-spread-functions (PSFs) recorded on a wide-field detector. However, optical aberrations distort those shapes, which compromises the accuracy and precision of single-molecule localization microscopy. Here, we employ a computational phase retrieval based on a vectorial PSF model to quantify the spatial variance of optical aberrations in a two-channel ultrawide-field single-molecule localization microscope. The use of a spatially variant PSF model enables accurate and precise emitter localization in x-, y- and z-directions throughout the entire field of view.