Open AccessStructured illumination imaging without grating rotation based on mirror operation on 1D Fourier spectrum
Author(s) -
Xin Jin,
Xumin Ding,
Jiubin Tan,
Xincheng Yao,
Cheng Shen,
Xuyang Zhou,
Cuimei Tan,
Shutian Liu,
Zhengjun Liu
Publication year - 2019
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.27.002016
Subject(s) - grating , optics , rotation (mathematics) , shadow (psychology) , fourier transform , physics , image quality , diffraction grating , resolution (logic) , computer science , artificial intelligence , image (mathematics) , psychology , quantum mechanics , psychotherapist
Structured illumination microscopy (SIM) is a rapidly developing a super-resolution optical microscopy technique. With SIM, the grating is needed in order to rotate several angles for illuminating the sample in different directions. Multiple rotations reduce the imaging speed and grating rotation angle errors damage the image recovery quality. We introduce mirror transformation on one-dimension (1D) Fourier spectrum to SIM for resolving the problems of low imaging speed and severe impact on image reconstruction quality by grating rotation angle errors. When mirror operation and SIM are combined, the grating is placed at an orientation for obtaining three shadow images. The three shadow images are acquired by CCD at three different phase shift for a direction of grating. Thus, the SIM imaging speed is faster and the effect on image reconstruction quality by grating rotation angle errors is greatly reduced.