Open AccessLow-cost, readily available 3D microscopy imaging system with variable focus spinner
Author(s) -
Lihui Wang,
Jianjiang Cui,
Satoshi Tabata,
Masatoshi Ishikawa
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.030576
Subject(s) - focus (optics) , optics , microscopy , depth of focus (tectonics) , depth of field , pixel , computer science , image resolution , cardinal point , aperture (computer memory) , computer vision , autofocus , artificial intelligence , materials science , physics , geology , acoustics , paleontology , subduction , tectonics
A large open aperture in an optical system can capture high-resolution images but yields a shallow depth of field. To overcome this issue, we investigated a low-cost, readily available method for retrofitting microscopy imaging systems to achieve 3D focus scanning in this study. Specifically, a procedure for fabricating variable focus spinners with dissimilar plates was introduced, and a sequence of 12 images was captured in different focal planes. The image scale and phase were corrected, and the in-focus pixels were abstracted by employing the Laplacian operator. Finally, an all-in-focus sharp image was generated, and a depth map was obtained.