
Fluorescence spectrum photo-bleaching analysis for distinguishing microorganisms (bacteria and fungi) from other particles in air
Author(s) -
Chenyang Lu,
Pei Zhang,
Shuanghong Chen,
JunJie Zhu,
Xiongli Xu,
Huijie Huang
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.028902
Subject(s) - fluorescence , microorganism , laser , optics , laser induced fluorescence , materials science , wavelength , bioaerosol , analytical chemistry (journal) , optoelectronics , chemistry , bacteria , physics , environmental chemistry , biology , aerosol , organic chemistry , genetics
A new device with a 405 nm blue laser diode is developed for collecting samples in air and detecting their spectra variation. The multi-sample particles which are pure microorganisms can be distinguished from interferents in the air by photo-bleaching phenomenon. Six types of microorganisms and twelve types of interferents from the air, which exhibit laser-induced fluorescence, are used to evaluate the performance of the analysis approach, and their fluorescence emission spectra are presented. The results show that when microorganisms are illuminated by the laser, the fluorescence spectra will change significantly within several minutes, including both the wavelength of the main peak and fluorescence intensity. Our work provides a potential approach to distinguish microorganisms from other particles by the changes.