Open AccessIntegration of stimulated Raman gain and stimulated Raman losses detection modes in a single nonlinear microscope
Author(s) -
Rajeev Ranjan,
Annalisa D’Arco,
Maria Antonietta Ferrara,
Maurizio Indolfi,
Michele Larobina,
L. Sirleto
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.026317
Subject(s) - femtosecond , raman spectroscopy , raman scattering , raman microscope , optics , materials science , microscopy , microscope , nanophotonics , laser , coherent anti stokes raman spectroscopy , optoelectronics , optical microscope , nonlinear optics , scanning electron microscope , physics
This work addresses some key challenges in the fields of bio and nanophotonics by stimulated Raman microscopy. We present the design and the implementation of a femtosecond stimulated Raman scattering microscope, equipped with three femtosecond laser sources, which allows the coexistence of stimulated Raman gain (SRG) and stimulate Raman losses (SRL) detection modes in a single microscopy setup and to generate images of the same region in succession, without adding or removing components. In order to demonstrate the switching between the two detection modes, SRL and SRG images of polystyrene beads are acquired and the images quality are evaluated and compared.