Open AccessCalibration of an in-water multi-excitation fluorometer for the measurement of phytoplankton chlorophyll-a fluorescence quantum yield
Author(s) -
D. Griffith,
Emma Lewis Bone,
Sandy Thomalla,
Stewart Bernard
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.018863
Subject(s) - fluorometer , quantum yield , fluorescence , calibration , optics , yield (engineering) , phytoplankton , population , excitation , attenuation coefficient , materials science , analytical chemistry (journal) , remote sensing , environmental science , physics , chemistry , chromatography , geology , demography , organic chemistry , quantum mechanics , sociology , nutrient , metallurgy
A multi-excitation fluorometer (MFL, JFE Advantech Co., Ltd.), originally designed to discriminate between phytoplankton species present within a population, has been redirected for use in fluorescence quantum yield (FQY) determination. While this calibration for apparent FQY requires no modification of the MFL, it is necessary to have an independent measurement of the spectral absorption coefficient of the subject fluid. Two different approaches to calibration were implemented. The primary method made use of reference fluorescent dye solutions of known quantum yield. The second method made use of acrylic fluorescent plaques and films. The two methods yielded consistent results, except in the 570 and 590 nm LED channels of the MFL. Application of the MFL in FQY determination is illustrated with an in situ Southern Ocean sample.