Open AccessQuantitative phase imaging system with slightly-off-axis configuration and suitable for objects both larger and smaller than the size of the image sensor
Author(s) -
Yang Yang,
Zhen-Jia Cheng,
Huimin Zhao,
Qing-Yang Yue,
Chen Guo
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.017199
Subject(s) - optics , holography , beam splitter , optical axis , reference beam , aperture (computer memory) , spatial frequency , magnification , phase (matter) , field of view , spatial filter , image plane , microscope , image resolution , physics , beam (structure) , computer science , lens (geology) , acoustics , image (mathematics) , computer vision , laser , quantum mechanics
We propose a quantitative phase imaging system with exact slightly-off-axis configuration and suitable for objects both smaller and larger than the size of the effective recording region of the image sensors. In this system, the object is illuminated by a convergent spherical beam and a specially designed aperture filter is placed on the spatial frequency plane of the object wave; at the same time, a point source emitting from the edge of the aperture is taken as the reference beam, so that the optimal frequency condition for reconstruction of slightly-off-axis digital holograms can be always guaranteed for both large and small objects as well as different magnification (or the field of view) configurations. At the same time, a 1x2 single-mode optical fiber splitter is used for generating the reference and the illumination beams. Benefited from such fiber-based slightly-off-axis design, the proposed system provides a low-cost way to convert a regular microscope into a slightly-off-axis holographic one for microbiological specimens with a high spatial resolution.