Open AccessImproving axial resolution for holographic tracking of colloids and bacteria over a wide depth of field by optimizing different factors
Author(s) -
Gui Huang,
Wenzhang Tian,
Qingda Meng,
Xiangjun Gong,
Guangzhao Zhang
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.009920
Subject(s) - optics , holography , tracking (education) , pixel , digital holographic microscopy , resolution (logic) , image resolution , depth of field , gaussian , materials science , physics , computer science , artificial intelligence , psychology , pedagogy , quantum mechanics
Improving the axial resolution for multiparticle three-dimensional (3D) holographic tracking is crucial but challenging. Here we study the impacts of incident light power, uniformity of the illumination as well as image pixel size on the axial tracking resolution for digital holographic microscopy (DHM). We demonstrate that the resolution highly depends on the image pixel size and the uniformity of the illumination. A 3D localization algorithm based on local-intensity-maxima searching and a Gaussian fit to the integrated intensity of the reconstructed lateral images along the axial direction proves a robust strategy to enhance the axial resolution for colloids and bacteria within a wide depth of field over several tens of micrometers.