Open AccessOptimal integration of wide field illumination and holographic optical tweezers for multimodal microscopy with ultimate flexibility and versatility
Author(s) -
Sang Hyuk Lee
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.008049
Subject(s) - holography , optics , optical tweezers , digital holographic microscopy , microscopy , microscope , light sheet fluorescence microscopy , fluorescence microscope , laser , interference microscopy , optical microscope , materials science , physics , scanning confocal electron microscopy , fluorescence , scanning electron microscope
We introduce one-of-a-kind optical microscope that we have developed through optimized integration of wide-field and focused-light microscopies. This new instrument has accomplished operation of the same laser for both wide field illumination and holographic focused beam illumination interchangeably or simultaneously in a way scalable to multiple lasers. We have demonstrated its powerful capability by simultaneously carrying out Epi-fluorescence, total internal reflection fluorescence microscopy, selective plane illumination microscopy, and holographic optical tweezers with five lasers. Our instrument and the optical design will provide researchers across diverse fields, cell-biology and biophysics in particular, with a practical guidance to build an all-around multimodal microscope that will further inspire the development of novel hybrid microscopy experiments.