Open AccessFluorescence emission difference with surface plasmon-coupled emission applied in confocal microscopy
Author(s) -
Kang Jiang,
Xiang Lei,
Kuanguo Li,
Yonghua Lu,
Pei Wang
Publication year - 2018
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.26.002380
Subject(s) - optics , materials science , fluorescence , surface plasmon , microscopy , point spread function , confocal , surface plasmon polariton , plasmon , confocal microscopy , microscope , fluorescence microscope , rayleigh scattering , polarization (electrochemistry) , physics , chemistry
We combined confocal surface plasmon coupled emission microscopy (C-SPCEM) together with fluorescence emission difference (FED) technique to pursuit super-resolution fluorescent image. Solid or hollow point spread function (PSF) for C-SPCEM is achieved with radially-polarized or circularly-polarized illumination. The reason why PSF can be manipulated by the polarization of illumination light is corroborated by the interaction of fluorescent emitter with vector focal field on the plasmonic substrate. After introduction of FED technique, PSF for C-SPECM can shrunk to around λ/4 in full-width half-maximum, which is unambiguously beyond Rayleigh's diffraction limit. The super-resolution capability of C-SPCEM with FED technique is experimentally demonstrated by imaging aggregated fluorescent beads with 150 nm in diameter.