Open AccessSimultaneous two-color imaging in digital holographic microscopy
Author(s) -
Nicola Farthing,
Rachel Christina Findlay,
Jan F. Jikeli,
Pegine Walrad,
M. A. Bees,
Laurence G. Wilson
Publication year - 2017
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.25.028489
Subject(s) - optics , digital holographic microscopy , microscopy , holography , digital holography , scattering , magnification , wavelength , materials science , light scattering , physics
We demonstrate the use of two-color digital holographic microscopy (DHM) for imaging microbiological subjects. The use of two wavelengths significantly reduces artifacts present in the reconstructed data, allowing us to image weakly-scattering objects in close proximity to strongly-scattering objects. We demonstrate this by reconstructing the shape of the flagellum of a unicellular eukaryotic parasite Leishmania mexicana in close proximity to a more strongly-scattering cell body. Our approach also yields a reduction of approximately one third in the axial position uncertainty when tracking the motion of swimming cells at low magnification, which we demonstrate with a sample of Escherichia coli bacteria mixed with polystyrene beads. The two-wavelength system that we describe introduces minimal additional complexity into the optical system, and provides significant benefits.