Open AccessOptical sectioning in optical resolution photo acoustic microscopy
Author(s) -
Seong-Ho Park,
Jean-Claude Vial,
Kwangseuk Kyhm
Publication year - 2017
Publication title -
optics express
Language(s) - Slovenian
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.25.018917
Subject(s) - optics , materials science , microscopy , resolution (logic) , absorption (acoustics) , photoacoustic effect , laser , rhodamine , optical microscope , acoustic microscopy , microscope , optoelectronics , fluorescence , physics , scanning electron microscope , artificial intelligence , computer science
We report a novel optical resolution photoacoustic microscopy concept to obtain an axial resolution only by optical methods. The photoacoustic signal is generated through a non-radiative relaxation from a level that is populated by excited state absorption. This two-step excitation process of a single laser enables to achieve an optical sectioning without any acoustic selectivity, whereby a full optical resolution photoacoustic microscopy is obtained. We bring a proof of this concept using Rhodamine and Zinc Tetraphenylporphyrin dyes known for their efficient excited state absorption process.