Open AccessDifferentiation of normal and leukemic cells by 2D light scattering label-free static cytometry
Author(s) -
Linyan Xie,
Qiao Li,
Changshun Shao,
Xuantao Su
Publication year - 2016
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.24.021700
Subject(s) - cytometry , light scattering , scattering , flow cytometry , optics , materials science , physics , medicine , immunology
Two-dimensional (2D) light scattering patterns of single microspheres, normal granulocytes and leukemic cells are obtained by label-free static cytometry. Statistical results of experimental 2D light scattering patterns obtained from standard microspheres with a mean diameter of 4.19 μm agree well with theoretical simulations. High accuracy rates (greater than 92%) for label-free differentiation of normal granulocytes and leukemic cells, both the acute and chronic leukemic cells, are achieved by analyzing the 2D light scattering patterns. Our label-free static cytometry is promising for leukemia screening in clinics.