Open AccessSuper-sensitivity multiphoton frequency-domain fluorescence lifetime imaging microscopy
Author(s) -
Yide Zhang,
Aamir A. Khan,
Genevieve D. Vigil,
Scott S. Howard
Publication year - 2016
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.24.020862
Subject(s) - fluorescence lifetime imaging microscopy , optics , microscopy , materials science , sensitivity (control systems) , microscope , fluorescence , fluorescence microscope , signal to noise ratio (imaging) , biological imaging , physics , electronic engineering , engineering
We present a series of experiments that demonstrate a super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime imaging microscopy (MPM-FD-FLIM) that shows a 2× improvement in imaging speed compared to the theoretical limit of conventional MPM-FD-FLIM. Additionally, this technique produces unprecedented sensitivity over a large range of fluorescence lifetimes. These results are achieved through simple modifications to data analysis in a conventional MPM-FD-FLIM microscope and are based on an analytical model describing the signal-to-noise ratio (SNR) of a MPM-FD-FLIM system [J. Opt. Soc. Am. A33, B1 (2016)]. Here we experimentally validate this model.