Open AccessHigh-dynamic-range fluorescence molecular tomography for imaging of fluorescent targets with large concentration differences
Author(s) -
Lichao Lian,
Yong Deng,
Wenhao Xie,
Guoqiang Xu,
Xiaoquan Yang,
Zhihong Zhang,
Qingming Luo
Publication year - 2016
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.24.019920
Subject(s) - fluorescence , imaging phantom , optics , dynamic range , tomography , fluorescence lifetime imaging microscopy , iterative reconstruction , high dynamic range , projection (relational algebra) , diffuse optical imaging , materials science , biomedical engineering , computer science , computer vision , physics , algorithm , medicine
When CCD-based free-space fluorescence molecular tomography (FMT) is used for imaging of fluorescent targets with a large concentration difference, the limited dynamic range of the CCD diminishes the localization and quantitative accuracy of FMT. To overcome this, we present a high-dynamic-range FMT (HDR-FMT) method. Under the multiple-exposure scheme, HDR fluorescence projection images are constructed using the recovered CCD response curve. Image reconstruction is implemented using iterative reweighted L1 regularization which can reduce artifacts by using fewer HDR fluorescence projection images. Phantom and in vivo animal studies indicate that localization of fluorescent targets with a large concentration difference is effectively improved with HDR-FMT and with good quantitative accuracy.