Open AccessLabel-free cellular structure imaging with 82 nm lateral resolution using an electron-beam excitation-assisted optical microscope
Author(s) -
Masahiro Fukuta,
Yoshihiro Masuda,
Wataru Inami,
Yoshimasa Kawata
Publication year - 2016
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.24.016487
Subject(s) - optics , microscope , microscopy , materials science , resolution (logic) , electron microscope , optical microscope , image resolution , optical sectioning , protein filament , fluorescence microscope , excitation , beam (structure) , scanning electron microscope , fluorescence , physics , composite material , quantum mechanics , artificial intelligence , computer science
We present label-free and high spatial-resolution imaging for specific cellular structures using an electron-beam excitation-assisted optical microscope (EXA microscope). Images of the actin filament and mitochondria of stained HeLa cells, obtained by fluorescence and EXA microscopy, were compared to identify cellular structures. Based on these results, we demonstrated the feasibility of identifying label-free cellular structures at a spatial resolution of 82 nm. Using numerical analysis, we calculated the imaging depth region and determined the spot size of a cathodoluminescent (CL) light source to be 83 nm at the membrane surface.