Open AccessIn vivo volumetric imaging of biological dynamics in deep tissue via wavefront engineering
Author(s) -
Lingjie Kong,
Juliet D. Tang,
Meng Cui
Publication year - 2016
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.24.001214
Subject(s) - wavefront , strehl ratio , optics , preclinical imaging , deformable mirror , image quality , lens (geology) , adaptive optics , materials science , physics , computer science , in vivo , computer vision , biology , microbiology and biotechnology , image (mathematics)
Biological systems undergo dynamical changes continuously which span multiple spatial and temporal scales. To study these complex biological dynamics in vivo, high-speed volumetric imaging that can work at large imaging depth is highly desired. However, deep tissue imaging suffers from wavefront distortion, resulting in reduced Strehl ratio and image quality. Here we combine the two wavefront engineering methods developed in our lab, namely the optical phase-locked ultrasound lens based volumetric imaging and the iterative multiphoton adaptive compensation technique, and demonstrate in vivo volumetric imaging of microglial and mitochondrial dynamics at large depth in mouse brain cortex and lymph node, respectively.