Open AccessFive-lens, easy-to-implement miniature objective for a fluorescence confocal microendoscope
Author(s) -
Yang Li,
Jiafu Wang,
Geng Tian,
Jing Yuan,
Qian Liu,
Ling Fu
Publication year - 2016
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.24.000473
Subject(s) - confocal , optics , confocal microscopy , lens (geology) , microscope , fluorescence , materials science , fluorescence microscope , fluorescence lifetime imaging microscopy , light sheet fluorescence microscopy , physics
A fluorescence confocal microendoscope requires a high-performance miniature objective. We present a miniature objective comprising four glass lenses and one plastic aspheric lens. The 0.5 NA objective is achromatized in the wavelength range of 488-550 nm, has a field of view (FOV) of 360 μm, and an outer diameter of 2.6 mm. The assembled miniature objective can resolve features separated by as little as 0.78 μm. The imaging quality of the fluorescence confocal microendoscope with the miniature objective is similar to that of a commercial confocal microscope. It can resolve cellular structures such as crypt structures and epithelial cells.