Open AccessSpectrometer-free biological detection method using porous silicon microcavity devices
Author(s) -
Peng Li,
Zhenhong Jia,
Xihong Lu,
Yajun Liu,
Xuefei Ning,
Jiaqing Mo,
Jiajia Wang
Publication year - 2015
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.024626
Subject(s) - spectrometer , optics , materials science , refractive index , porous silicon , detection limit , silicon , resolution (logic) , light intensity , optoelectronics , computer science , physics , chemistry , chromatography , artificial intelligence
This paper proposes a label-free and spectrometer-free method for biological detection with high detecting resolution. Taking advantage of the optical properties of porous silicon microcavity, the refractive index changes caused by biological reaction can be detected by measuring the incident angle of the minimum reflected light intensity. Based on the above method, label-free eight-base pair DNA detection can be realized with a corresponding detection limit is as low as 87 nM. This method provides high detecting resolution at a low equipment cost, and can be further used to develop an advanced instrument for biological detection.