Open AccessMicro-anatomical and functional assessment of ciliated epithelium in mouse trachea using optical coherence phase microscopy
Author(s) -
Rehman Ansari,
Christian Buj,
Mario Pieper,
Peter König,
Achim Schweikard,
Gereon Hüttmann
Publication year - 2015
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.023217
Subject(s) - optical coherence tomography , cilium , microscopy , optics , confocal microscopy , phase contrast microscopy , coherence (philosophical gambling strategy) , confocal , optical microscope , materials science , biology , physics , microbiology and biotechnology , scanning electron microscope , quantum mechanics
Motile cilia perform a range of important mechanosensory and chemosensory functions, along with expulsion of mucus and inhaled pathogens from the lungs. Here we demonstrate that spectral domain optical coherence phase microscopy (SD-OCPM), which combines the principles of optical coherence tomography (OCT) and confocal microscopy, is particularly well-suited for characterization of both morphology and the ciliary dynamics of mouse trachea. We present micro-anatomical images of mouse trachea, where different cell types can be clearly visualized. The phase contrast, which measures the sub-nanometer changes in axial optical pathlength is used to determine the frequency and direction of cilia beatings.