Open AccessNew modes in label-free super resolution based on photo-modulated reflectivity
Author(s) -
Omer Tzang,
Ori Cheshnovsky
Publication year - 2015
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.020926
Subject(s) - optics , modulation (music) , materials science , reflectivity , nonlinear system , image resolution , microscopy , spatial frequency , resolution (logic) , laser , frequency modulation , computer science , physics , radio frequency , telecommunications , artificial intelligence , acoustics , quantum mechanics
The recent advances in far-field super-resolution (SR) microscopy rely on, and therefore are limited by the ability to control the fluorescence of label molecules. We demonstrated a far field label-free SR methodology that relies on the nonlinear response of the reflectance to photo-modulation by a pump laser. Here we extend our approach in two directions. We show that the method can be further simplified and improved by using a single beam rather than a pump and probe or by adding spatial probe modulation to improve resolution. Additionally, we demonstrate SR in sectioning and further investigate the dynamics of non-linearity in photo-modulated reflectance. These new modalities of nonlinear photo-modulated reflectivity (NPMR) enhance its applicability using lower orders of nonlinear response.