Open AccessOpen-top selective plane illumination microscope for conventionally mounted specimens
Author(s) -
Ryan McGorty,
Harrison Liu,
Daichi Kamiyama,
Zhiqiang Dong,
Su Guo,
Bo Huang
Publication year - 2015
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.016142
Subject(s) - microscope , microfluidics , optics , prism , microscopy , materials science , inverted microscope , optical microscope , light sheet fluorescence microscopy , live cell imaging , nanotechnology , biology , scanning electron microscope , physics , scanning confocal electron microscopy , genetics , cell
We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips.