Open-top selective plane illumination microscope for conventionally mounted specimens | Zendy

Ryan McGorty | Zendy; Harrison Liu | Zendy; Daichi Kamiyama | Zendy; Zhiqiang Dong | Zendy; Su Guo | Zendy; Bo Huang | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Open-top selective plane illumination microscope for conventionally mounted specimens

Author(s) -

Ryan McGorty,

Harrison Liu,

Daichi Kamiyama,

Zhiqiang Dong,

Su Guo,

Bo Huang

Publication year - 2015

Publication title -

optics express

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.23.016142

Subject(s) - microscope , optics , microfluidics , prism , materials science , microscopy , inverted microscope , optical microscope , nanotechnology , scanning electron microscope , physics

We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research