Open AccessLensless phase microscopy and diffraction tomography with multi-angle and multi-wavelength illuminations using a LED matrix
Author(s) -
Chao Zuo,
Jiasong Sun,
Jialin Zhang,
Yan Hu,
Qian Chen
Publication year - 2015
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.014314
Subject(s) - optics , diffraction , materials science , tomography , diffraction tomography , microscopy , optical coherence tomography , phase retrieval , resolution (logic) , image resolution , wavelength , optoelectronics , computer science , physics , artificial intelligence , fourier transform , quantum mechanics
We demonstrate lensless quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED matrix. Based on the multi-wavelength phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of 3.72μm and an axial resolution of 5μm, across a wide field-of-view of 24mm 2 . We experimentally demonstrate the success of our method by imaging cheek cells, micro-beads, and fertilized eggs of Parascaris equorum. Such high-throughput and miniaturized imaging device can provide a cost-effective tool for telemedicine applications and point-of-care diagnostics in resource-limited environments.