Open AccessResolution-enhanced surface plasmon-coupled emission microscopy
Author(s) -
Baoliang Ge,
Ye Ma,
Cuifang Kuang,
Douguo Zhang,
Kimani C. Toussaint,
Shangting You,
Xu Liu
Publication year - 2015
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.013159
Subject(s) - optics , surface plasmon , materials science , diaphragm (acoustics) , image resolution , microscopy , resolution (logic) , light sheet fluorescence microscopy , surface plasmon resonance , distortion (music) , surface plasmon polariton , plasmon , physics , optoelectronics , scanning confocal electron microscopy , nanotechnology , computer science , amplifier , acoustics , cmos , artificial intelligence , loudspeaker , nanoparticle
A novel fluorescence emission difference technique is proposed for further enhancements of the lateral resolution in surface plasmon-coupled emission microscopy (SPCEM). In the proposed method, the difference between the image with phase modulation by using a 0-2π vortex phase plate (VPP) along with a diaphragm and the original image obtained from SPCEM is used to estimate the spatial distribution of the analyzed sample. By optimizing the size of the diaphragm and the subtractive factor, the lateral resolution can be enhanced by about 20% and 33%, compared with that in SPCEM with a single 0-2π VPP and conventional wide-field fluorescence microscopy, respectively. Related simulation results are presented to verify the capability of the proposed method for improving lateral resolution and reducing imaging distortion. It is believed that the proposed method has potentials to improve the performance of SPCEM, thus facilitating biological observation and research.