Open AccessDetermining the orientation of transition moments and depolarization by fluorescence polarizing angle spectrum
Author(s) -
Taotao Mu,
Siying Chen,
Yinchao Zhang,
Pan Guo,
He Chen
Publication year - 2015
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.011748
Subject(s) - depolarization , polarization (electrochemistry) , optics , degree of polarization , dipole , excitation , fluorescence , orientation (vector space) , materials science , physics , brewster's angle , transition dipole moment , chemistry , geometry , mathematics , biophysics , scattering , brewster , quantum mechanics , biology
In this paper, fluorescence polarizing angle spectrum, combined with degree of polarization(DOP), is proposed to determine the spatial orientation of transition dipole moments (TDMs) and depolarization of chlorophyll in solution. It is found that, due to the oriented TDMs under polarized laser excitation, the projections of angle of polarization(AOP) and DOP on the three orthogonal planes are different from each other. Experiments demonstrate that we can acquire the spatial orientation by detecting the projections of AOP on two orthogonal planes (xOz and yOz). Meanwhile, The depolarization can also be determined by the DOP spectrums. The validity of this method has been verified by another projection on the xOy plane.