Open AccessCompact light source for ultrabroadband coherent anti-Stoke Raman scattering (CARS) microscopy
Author(s) -
Hideharu Mikami,
Manabu Shiozawa,
Masamitsu Shirai,
Keiji Watanabe
Publication year - 2015
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.23.002872
Subject(s) - optics , microscopy , raman scattering , laser , materials science , raman spectroscopy , nanosecond , light scattering , polarization (electrochemistry) , photonic crystal fiber , fiber laser , biophotonics , optoelectronics , scattering , optical fiber , physics , chemistry
A compact light source module for ultrabroadband coherent anti-Stoke Raman scattering (CARS) microscopy was developed. It mainly consists of a nanosecond microchip laser, a photonic crystal fiber for Stokes light generation, and a single mode polarization maintaining fiber for pump light propagation. It is alignment-free and relatively low-cost compared with previous light sources of CARS microscopy. By using an assembled module, we successfully observed an ultrabroadband CARS spectrum and a CARS image of a murine adipocyte. The module is expected to greatly spread the CARS microscopy to various fields by its extreme easiness to handle.