Open AccessMultimodal nonlinear optical microscopy with shaped 10 fs pulses
Author(s) -
Jean Rehbinder,
Lukas Brückner,
Alexander Wipfler,
Tiago Buckup,
Marcus Motzkus
Publication year - 2014
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.028790
Subject(s) - optics , raman scattering , second harmonic generation , microscopy , materials science , nonlinear optics , high harmonic generation , self phase modulation , nonlinear system , pulse shaping , raman spectroscopy , laser , optoelectronics , physics , quantum mechanics
We demonstrate the use of shaped 10 fs pulses for multimodal microscopy. The combination of a broadband oscillator and a pulse shaper provides a flexible light source that can be optimized for various nonlinear effects produced in the sample, either for signal intensity or for selectivity. While the highest nonlinear generation efficiency is achieved with the shortest pulses, more complex waveforms address specific transitions in the sample for better contrast. This is shown experimentally with the imaging of a moss leaf and of human skin biopsies using coherent anti-Stokes Raman scattering, two-photon fluorescence and second harmonic generation signals.