Open AccessTwo-photon excitation STED microscopy by utilizing transmissive liquid crystal devices
Author(s) -
Kohei Otomo,
Terumasa Hibi,
Yuichi Kozawa,
Makoto Kurihara,
Nobuyuki Hashimoto,
Hiroyuki Yokoyama,
Shunichi Sato,
Tomomi Nemoto
Publication year - 2014
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.028215
Subject(s) - sted microscopy , optics , materials science , microscopy , microscope , optical path , liquid crystal , two photon excitation microscopy , polarization (electrochemistry) , excitation , optoelectronics , laser , wavelength , biophotonics , optical microscope , super resolution microscopy , stimulated emission , physics , fluorescence , scanning confocal electron microscopy , scanning electron microscope , chemistry , quantum mechanics
Transmissive liquid crystal devices (tLCDs) enable the modification of optical properties, such as phase, polarization, and laser light intensity, over a wide wavelength region at a high conversion efficiency. By utilizing tLCDs, we developed a new two-photon excitation stimulated emission depletion microscopy technique based on a conventional two-photon microscope. Spatial resolution was improved by compensating for phase shifts distributed in the optical path. Using this technique, we observed the fine structures of microtubule networks in fixed biological cells.