Open AccessCharacterizing and tracking individual colloidal particles using Fourier-Bessel image decomposition
Author(s) -
Filip Strubbe,
Stijn Vandewiele,
Caspar Schreuer,
Filip Beunis,
Oksana Drobchak,
Toon Brans,
Kristiaan Neyts
Publication year - 2014
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.024635
Subject(s) - optics , fourier transform , materials science , refractive index , polystyrene , dispersity , brownian motion , microscopy , physics , polymer chemistry , polymer , quantum mechanics , composite material
We use Fourier-Bessel Image Decomposition (FBID) of microscopy images to investigate the size, refractive index and 3-dimensional position of individual colloidal microspheres. With measurements of monodisperse polystyrene and poly(methyl methacrylate) particles we achieve a resolution of 1% in size and 0.2% in refractive index for a single image which is sufficient for accurate in situ characterization of polydisperse colloids. Also the binding of avidin molecules to individual biotinylated polystyrene particles is resolved. Finally, the FBID method offers a straightforward approach to 3-dimensional out-of-focus tracking. Here, the z-position of a freely diffusing particle is calculated by applying the statistics of Brownian motion to its set of Fourier-Bessel coefficients.