Open AccessIn vivo fluorescence microscopy via iterative multi-photon adaptive compensation technique
Author(s) -
Lingjie Kong,
Meng Cui
Publication year - 2014
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.023786
Subject(s) - optics , two photon excitation microscopy , wavefront , microscopy , preclinical imaging , adaptive optics , compensation (psychology) , in vivo , materials science , photon counting , fluorescence lifetime imaging microscopy , deformable mirror , fluorescence microscope , fluorescence , photon , physics , biology , psychology , microbiology and biotechnology , psychoanalysis
Iterative multi-photon adaptive compensation technique (IMPACT) has been developed for wavefront measurement and compensation in highly scattering tissues. Our previous report was largely based on the measurements of fixed tissue. Here we demonstrate the advantages of IMPACT for in vivo imaging and report the latest results. In particular, we show that IMPACT can be used for functional imaging of awake mice, and greatly improve the in vivo neuron imaging in mouse cortex at large depth (~660 microns). Moreover, IMPACT enables neuron imaging through the intact skull of adult mice, which promises noninvasive optical measurements in mouse brain.