Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy | Zendy

Nisan Siegel | Zendy; Gary Brooker | Zendy

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Improved axial resolution of FINCH fluorescence microscopy when combined with spinning disk confocal microscopy

Author(s) -

Nisan Siegel,

Gary Brooker

Publication year - 2014

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.22.022298

Subject(s) - optics , confocal , confocal microscopy , microscopy , materials science , digital holographic microscopy , fluorescence microscope , light sheet fluorescence microscopy , focus (optics) , resolution (logic) , scanning confocal electron microscopy , scanner , super resolution microscopy , microscope , holography , fluorescence lifetime imaging microscopy , fluorescence , physics , computer science , artificial intelligence

FINCH holographic fluorescence microscopy creates super-resolved images with enhanced depth of focus. Addition of a Nipkow disk real-time confocal image scanner is shown to reduce the FINCH depth of focus while improving transverse confocal resolution in a combined method called "CINCH".

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