Open AccessPhotoacoustic microscopy using ultrashort pulses with two different pulse durations
Author(s) -
Yoshihisa Yamaoka,
Yoshinori Harada,
Masaaki Sakakura,
Takeo Minamikawa,
Shigeru Nishino,
Seiji Maehara,
Shujiro Hamano,
Hideo Tanaka,
Tetsuro Takamatsu
Publication year - 2014
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.017063
Subject(s) - optics , two photon excitation microscopy , femtosecond pulse shaping , ultrashort pulse , materials science , femtosecond , picosecond , absorption (acoustics) , pulse (music) , photoacoustic doppler effect , microscopy , photon , two photon absorption , pulse duration , photoacoustic effect , pulse shaping , laser , fluorescence , physics , detector
We propose photoacoustic microscopy using ultrashort pulses with two different pulse durations in the range from femtoseconds to picoseconds. The subtraction of images for longer-pulse excitation from those for shorter-pulse excitation extracts two-photon photoacoustic images effectively, based on observation that the intensity ratio of two-photon to one-photon absorption-induced photoacoustic signals depends on the pulse duration in the same manner as the intensity ratio of two-photon and one-photon fluorescence signals. Two-photon photoacoustic microscopy using this subtraction method enables precise observation of the cross-sections of silicone hollows filled with the mixture of one-photon and two-photon absorption solutions.