Photoacoustic microscopy using ultrashort pulses with two different pulse durations | Zendy

Yoshihisa Yamaoka | Zendy; Yoshinori Harada | Zendy; Masaaki Sakakura | Zendy; Takeo Minamikawa | Zendy; Shigeru Nishino | Zendy; Seiji Maehara | Zendy; Shujiro Hamano | Zendy; Hideo Tanaka | Zendy; Tetsuro Takamatsu | Zendy

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Photoacoustic microscopy using ultrashort pulses with two different pulse durations

Author(s) -

Yoshihisa Yamaoka,

Yoshinori Harada,

Masaaki Sakakura,

Takeo Minamikawa,

Shigeru Nishino,

Seiji Maehara,

Shujiro Hamano,

Hideo Tanaka,

Tetsuro Takamatsu

Publication year - 2014

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.22.017063

Subject(s) - optics , two photon excitation microscopy , femtosecond pulse shaping , ultrashort pulse , materials science , femtosecond , picosecond , absorption (acoustics) , pulse (music) , photoacoustic doppler effect , microscopy , photon , two photon absorption , pulse duration , photoacoustic effect , pulse shaping , laser , fluorescence , physics , detector

We propose photoacoustic microscopy using ultrashort pulses with two different pulse durations in the range from femtoseconds to picoseconds. The subtraction of images for longer-pulse excitation from those for shorter-pulse excitation extracts two-photon photoacoustic images effectively, based on observation that the intensity ratio of two-photon to one-photon absorption-induced photoacoustic signals depends on the pulse duration in the same manner as the intensity ratio of two-photon and one-photon fluorescence signals. Two-photon photoacoustic microscopy using this subtraction method enables precise observation of the cross-sections of silicone hollows filled with the mixture of one-photon and two-photon absorption solutions.

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