Open AccessA method for super-resolved CARS microscopy with structured illumination in two dimensions
Author(s) -
Joo Hyun Park,
SangWon Lee,
Eun Seong Lee,
Jae Yong Lee
Publication year - 2014
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.009854
Subject(s) - optics , microscopy , raman scattering , diffraction , image resolution , spatial frequency , physics , resolution (logic) , raman spectroscopy , computer science , artificial intelligence
We propose a structured illumination scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy with a resolution surpassing the diffraction limit in two dimensions (2D). By acquiring a set of coherent images of a sample with third-order nonlinear susceptibility illuminated by the phase-matched excitation field of square lattice patterns, a 2D super-resolution CARS image can be reconstructed. We derive a theoretical framework to describe the coherent image formation and reconstruction scheme for this structured illumination CARS imaging system and carry out numerical simulations to investigate its imaging performance. The results demonstrate that our method promises a particular benefit on CARS microscopy by adding the super-resolution capability to improve its 2D spatial resolution by a factor of approximately three.