Open AccessIn vivo imaging of middle-ear and inner-ear microstructures of a mouse guided by SD-OCT combined with a surgical microscope
Author(s) -
Nam Hyun Cho,
Jeong Hun Jang,
Woonggyu Jung,
Jeehyun Kim
Publication year - 2014
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.22.008985
Subject(s) - optical coherence tomography , microscope , middle ear , eyepiece , optics , operating microscope , preclinical imaging , microscopy , optical microscope , materials science , biomedical engineering , in vivo , medicine , anatomy , scanning electron microscope , biology , lens (geology) , physics , microbiology and biotechnology
We developed an augmented-reality system that combines optical coherence tomography (OCT) with a surgical microscope. By sharing the common optical path in the microscope and OCT, we could simultaneously acquire OCT and microscope views. The system was tested to identify the middle-ear and inner-ear microstructures of a mouse. Considering the probability of clinical application including otorhinolaryngology, diseases such as middle-ear effusion were visualized using in vivo mouse and OCT images simultaneously acquired through the eyepiece of the surgical microscope during surgical manipulation using the proposed system. This system is expected to realize a new practical area of OCT application.