Open AccessDengue immunoassay with an LSPR fiber optic sensor
Author(s) -
Alexandre R. Camara,
Paula M. P. Gouvêa,
Ana Carolina M. S. Dias,
Arthur M. B. Braga,
Rosa F. Dutra,
Renato E. de Araujo,
Isabel C. S. Carvalho
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.027023
Subject(s) - dengue fever , surface plasmon resonance , materials science , fiber optic sensor , optical fiber , colloidal gold , immunoassay , specular reflection , optics , fiber , virology , nanotechnology , nanoparticle , medicine , antibody , immunology , composite material , physics
Dengue fever is a viral disease that affects millions of people worldwide. Specific tests for dengue are not usually performed due to high costs, complicated procedures and, in some cases, long time to yield a result. For widespread use of specific tests to be possible, fast, reliable and fairly simple methods are needed. In this paper, we present a new dengue diagnostic method for the acute phase of the infection. The method proposed uses an all-optical fiber sensor based on Localized Surface Plasmon Resonance (LSPR) and specular reflection from gold nanoparticles (AuNPs). Dengue anti-NS1 antibody was immobilized on AuNPs deposited on the endface of a standard multimode fiber (62.5 µm/125 µm). The sensor is able to detect NS1 antigen at different concentrations, with limit of quantification estimated to be 0.074 μg/ml = 1.54 nM. These results indicate that the sensor could potentially be used for dengue diagnosis in the acute phase of the infection.