Open AccessDegeneration of Fraunhofer diffraction on bacterial colonies due to their light focusing properties examined in the digital holographic microscope system
Author(s) -
Igor Buzalewicz,
Kamil Liżewski,
Małgorzata Kujawińska,
Halina Podbielska
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.026493
Subject(s) - optics , holography , digital holographic microscopy , diffraction , digital holography , microscope , diffraction efficiency , materials science , microscopy , fresnel diffraction , phase (matter) , physics , quantum mechanics
The degeneration of Fraunhofer diffraction conditions in the optical system with converging spherical wave illumination for bacteria species identification based on diffraction patterns is analyzed by digital holographic methods. The obtained results have shown that the colonies of analyzed bacteria species act as biological lenses with the time-dependent light focusing properties, which are characterized and monitored by means of phase retrieval from sequentially captured digital holograms. This significantly affects the location of Fraunhofer patterns observation plane, which is continuously shifted across optical axis in time.