
Reflection-mode optical-resolution photoacoustic microscopy based on a reflective objective
Author(s) -
Hui Wang,
Xiaoquan Yang,
Yanyan Liu,
Bowen Jiang,
Qingming Luo
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.024210
Subject(s) - optics , materials science , photoacoustic doppler effect , microscopy , transducer , reflection (computer programming) , ultrasonic sensor , penetration depth , ultrasound , microscope , total internal reflection , diffraction , photoacoustic imaging in biomedicine , optoelectronics , acoustics , physics , computer science , programming language
We developed a new reflection-mode optical-resolution photoacoustic microscopy (OR-PAM) based on the cooperation of a reflective objective and an ultrasonic transducer. The reflective objective is used to produce nearly diffraction-limited optical focusing, and the excited ultrasound waves are then directly detected by an ultrasonic transducer that was placed in the central cone of the objective. This new design avoids the coupling between optical focusing and ultrasound transmission in the reflection mode. Moreover, the proposed system is able to provide lateral resolution of 1.2 μm at 580 nm, penetration depth of 0.9 mm in biological tissues, and a work distance of 6.0 mm. We present in vivo imaging of the microvasculature in mouse ears and in vitro imaging of red blood cells (RBCs), which demonstrate the capability of the system to study microcirculation.