Open AccessA fast fluorescence imaging flow cytometer for phytoplankton analysis
Author(s) -
Jianglai Wu,
Robert K. Y. Chan
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.023921
Subject(s) - optics , deconvolution , phytoplankton , motion blur , materials science , temporal resolution , fluorescence , image resolution , remote sensing , environmental science , physics , geology , computer science , biology , image (mathematics) , computer vision , ecology , nutrient
We report a fast fluorescence imaging flow cytometer for phytoplankton analysis that can achieve a volume flow rate up to 1ml/min. The instrument shows a high immunity to motion blur in image captured with a lateral resolution of 0.75 ± 0.06 μm for a wide size range ~1 μm to ~200 μm. This is made possible by suppressing the out-of-focus light using thin light sheet illumination and image deconvolution, and by precluding the motion-blur with a unique flow configuration. Preliminary results from untreated coastal water samples show the technique has high potential as a practical field instrument for monitoring phytoplankton abundance and species composition.