Open AccessNear shot-noise limited hyperspectral stimulated Raman scattering spectroscopy using low energy lasers and a fast CMOS array
Author(s) -
William Rock,
Mischa Bonn,
Sapun H. Parekh
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.015113
Subject(s) - hyperspectral imaging , optics , raman scattering , shot noise , demodulation , spectroscopy , coherent anti stokes raman spectroscopy , raman spectroscopy , materials science , physics , noise (video) , laser , scattering , optoelectronics , telecommunications , computer science , detector , channel (broadcasting) , quantum mechanics , artificial intelligence , image (mathematics)
We demonstrate near shot-noise limited hyperspectral stimulated Raman scattering (SRS) spectroscopy using oscillator-only excitation conditions. Using a fast CMOS camera synchronized to an acousto-optic modulator and subtracting subsequent frames acquired at up to 1 MHz frame rates, we demonstrate demodulation and recovery of the SRS spectrum. Surprisingly, we observe that the signal-to-noise of SRS spectra is invariant at modulation frequencies down to 2.5 kHz. Our approach allows for a direct comparison of SRS with coherent anti-Stokes Raman scattering (CARS) spectroscopy under identical experimental conditions. Our findings suggest that hyperspectral SRS imaging with shot-noise limited performance at biologically compatible excitation energies is feasible after minor modifications to fast frame-rate CMOS array technology.