Open AccessSelective detection of sub-atto-molar Streptavidin in 10^13-fold impure sample using photonic crystal nanolaser sensors
Author(s) -
Shoji Hachuda,
Shota Otsuka,
Shota Kita,
Toshinari Isono,
Michimasa Narimatsu,
Keisuke Watanabe,
Yoshio Goshima,
Toshihiko Baba
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.012815
Subject(s) - streptavidin , nanolaser , biosensor , photonic crystal , detection limit , materials science , photonics , bovine serum albumin , optics , optoelectronics , nanotechnology , chemistry , biotin , chromatography , physics , wavelength , biochemistry , lasing threshold
Biosensors selectively detecting a very small amount of biomarker protein in human blood are desired for early and reliable diagnoses of severe diseases. This paper reports the detection of protein (streptavidin: SA) in ultra-low concentration, with an ultra-high selectivity against contaminants, using photonic crystal nanolasers. For biotin-modified nanolasers in pure water with SA, an extremely-low detection limit of 16 zM is evaluated. Even in a mixture with 1 μM bovine serum albumin as the contaminant, 100 zM SA is detected, meaning a selectivity of 10(13). These are remarkable capabilities that are promising for practical biosensing in the medical applications mentioned above.