Open AccessEnhancing diffractive multi-plane microscopy using colored illumination
Author(s) -
Alexander Jesacher,
Clemens Roider,
Monika RitschMarte
Publication year - 2013
Publication title -
optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.011150
Subject(s) - optics , diffraction , microscopy , cardinal point , chromatic aberration , spatial light modulator , reflection (computer programming) , ptychography , spatial frequency , chromatic scale , microscope , materials science , physics , computer science , programming language
We present a method to increase the number of simultaneously imaged focal planes in diffractive multi-plane imaging. We exploit the chromatic properties of diffraction by using multicolor LED illumination and demonstrate time-synchronous imaging of up to 21 focal planes.We discuss the possibilities and limits given by the use of a liquid crystal spatial light modulator to display the diffractive patterns. The method is suitable for wide-field transmission and reflection microscopy.