Enhancing diffractive multi-plane microscopy using colored illumination | Zendy

Alexander Jesacher | Zendy; Clemens Roider | Zendy; Monika RitschMarte | Zendy

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Enhancing diffractive multi-plane microscopy using colored illumination

Author(s) -

Alexander Jesacher,

Clemens Roider,

Monika RitschMarte

Publication year - 2013

Publication title -

optics express

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.21.011150

Subject(s) - optics , diffraction , microscopy , cardinal point , chromatic aberration , spatial light modulator , reflection (computer programming) , ptychography , spatial frequency , chromatic scale , microscope , materials science , physics , computer science , programming language

We present a method to increase the number of simultaneously imaged focal planes in diffractive multi-plane imaging. We exploit the chromatic properties of diffraction by using multicolor LED illumination and demonstrate time-synchronous imaging of up to 21 focal planes.We discuss the possibilities and limits given by the use of a liquid crystal spatial light modulator to display the diffractive patterns. The method is suitable for wide-field transmission and reflection microscopy.

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