z-logo
open-access-imgOpen Access
Two-photon excitation selective plane illumination microscopy (2PE-SPIM) of highly scattering samples: characterization and application
Author(s) -
Zeno Lavagnino,
Francesca Cella Zanacchi,
Emiliano Ronzitti,
Alberto Diaspro
Publication year - 2013
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.21.005998
Subject(s) - optics , imaging phantom , excitation , scattering , materials science , microscopy , two photon excitation microscopy , light scattering , photon , optoelectronics , physics , fluorescence , quantum mechanics
In this work we report the advantages provided by two photon excitation (2PE) implemented in a selective plane illumination microscopy (SPIM) when imaging thick scattering samples. In particular, a detailed analysis of the effects induced on the real light sheet excitation intensity distribution is performed. The comparison between single-photon and two-photon excitation profiles shows the reduction of the scattering effects and sample-induced aberrations provided by 2PE-SPIM. Furthermore, uniformity of the excitation distribution and the consequent improved image contrast is shown when imaging scattering phantom samples in depth by 2PE-SPIM. These results show the advantages of 2PE-SPIM and suggest how this combination can further enhance the SPIM performance. Phantom samples have been designed with optical properties compatible with biological applications of interest.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here