Open AccessLateral shearing digital holographic imaging of small biological specimens
Author(s) -
Amardeep Singh,
Arun Anand,
Rainer A. Leitgeb,
Bahram Javidi
Publication year - 2012
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.20.023617
Subject(s) - optics , shearing (physics) , materials science , phase imaging , interferometry , holography , holographic interferometry , digital holographic microscopy , shearing interferometer , digital holography , microfluidics , microscope , microscopy , astronomical interferometer , physics , nanotechnology , composite material
A lateral shearing interferometer is used for direct holographic imaging of microorganisms. This is achieved by increasing the shear to be larger than the object size and results in a very simple and inexpensive common-path imaging device that can be easily coupled to the output of an inverted microscope. The shear is created by reflections from the front and back surface of a glass plate. Stability measurements show a standard deviation of the phase measurements of less than 1nm over 8 min. without any vibration compensation. The setup is applied to imaging both microorganisms in a microfluidic channel and red blood cells and reconstructions are presented.