Lateral shearing digital holographic imaging of small biological specimens | Zendy

Amardeep Singh | Zendy; Arun Anand | Zendy; Rainer A. Leitgeb | Zendy; Bahram Javidi | Zendy

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Lateral shearing digital holographic imaging of small biological specimens

Author(s) -

Amardeep Singh,

Arun Anand,

Rainer A. Leitgeb,

Bahram Javidi

Publication year - 2012

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.20.023617

Subject(s) - optics , shearing (physics) , phase imaging , holographic interferometry , materials science , holography , interferometry , digital holographic microscopy , digital holography , shearing interferometer , microscope , microfluidics , interference microscopy , microscopy , astronomical interferometer , physics , nanotechnology , composite material

A lateral shearing interferometer is used for direct holographic imaging of microorganisms. This is achieved by increasing the shear to be larger than the object size and results in a very simple and inexpensive common-path imaging device that can be easily coupled to the output of an inverted microscope. The shear is created by reflections from the front and back surface of a glass plate. Stability measurements show a standard deviation of the phase measurements of less than 1nm over 8 min. without any vibration compensation. The setup is applied to imaging both microorganisms in a microfluidic channel and red blood cells and reconstructions are presented.

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