Open AccessImaging Fourier transform endospectroscopy for in vivo and in situ multispectral imaging
Author(s) -
Hongming Zhang,
Jing Yuan,
Ling Fu
Publication year - 2012
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.20.023349
Subject(s) - multispectral image , optics , preclinical imaging , materials science , spectral imaging , in vivo , in situ , fourier transform , image resolution , resolution (logic) , fluorescence lifetime imaging microscopy , biomedical engineering , spectral resolution , fourier transform spectroscopy , medical imaging , fluorescence , fourier transform infrared spectroscopy , remote sensing , chemistry , physics , computer science , medicine , artificial intelligence , geology , microbiology and biotechnology , quantum mechanics , astronomy , biology , spectral line , organic chemistry
We report the design and implementation of a multispectral imaging Fourier transform endospectroscopy (IFTES) system. The IFTES system employs a flexible fiber bundle catheter coupled to a home-built imaging Fourier transform spectroscope. The instrument enables the performance of non- or minimally invasive subsurface imaging and multispectral imaging at the cellular level in vivo and in situ. A maximum spectral resolution of 0.2 nm at 632.8 nm and a lateral resolution of 4.4 μm were proved. Preliminary results of a standard resolution target, ex-vivo small animal tissue, single wavelength laser, fluorescence solution, in-vivo mouse skin, microspheres mixture, and in-vivo transgenic mouse brain were given to demonstrate the potential of the technique.